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What is the purpose of incubating the plates?

Incubating the plates to promote growth of microbes is an essential part of any microbiology investigation. Incubating in aerobic conditions, and below human body temperature, reduce the risk of encouraging microorganisms (particularly bacteria) that could be pathogenic to humans.

Why were you asked to incubate the plates at two different temperatures?

The primary reason for incubating bacterial cultures at different temperatures is that specific bacteria are adapted to grow best at different temperatures.

How does growth on the unopened plates affect the reliability of the other plates?

How does growth on the unopened plates affect the reliability (your interpretation) of the other plates? Contamination when agar was plated could cause contamination in all or just one plate. Growth on unopened plates means the other plates could also be contaminated and the results are not sound.

Why must the mold plates be incubated for so much longer than the nutrient agar plates?

Why must the mold plates be incubated for so much longer than the nutrient agar plates? The molds grow much more slowly than bacteria. Extra time is required to inhibit bacterial growth on the plates. The molds grow much faster than bacteria, and the high heat inhibits this growth.

Why are the plates from this experiment incubated at room temperature 25 C?

Why are nutrient agar plates incubated at 37 degrees C and Sabouraud agar at 25 degrees C. Both optimum temps. 37 = human body temp = temp at which some of bodies bacteria will grow. 25 = optimum temp for fungi as low pH kills bacteria anyway.

What are the two conditions for bacterial growth that you can control?

Bacteria can live in hotter and colder temperatures than humans, but they do best in a warm, moist, protein-rich environment that is pH neutral or slightly acidic.

Where does bacteria grow the most?

Information. Bacteria grow most rapidly in the range of temperatures between 40 °F and 140 °F, doubling in number in as little as 20 minutes. This range of temperatures is often called the “Danger Zone.” To learn more about the “Danger Zone” visit the Food Safety and Inspection Service fact sheet titled Danger Zone.

In which condition will Bacteria grow rapidly?

danger zone