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Which is the correct order for the hydrolysis of sucrose reaction?

Given: The hydrolysis of sucrose into glucose and fructose in acidic water is a first-order reaction.

What is the reaction order with respect to sucrose?

Comparing experiments 1 and 2, concentration of sucrose increase by 1.5 times and there is no change in concentration of HCl. So the increase in initial rates by 1.5 times must be due to sucrose only. Since this is a proportionate increase, order of reaction with respect to sucrose is also order 1.

Which of the following is obtained on hydrolysis of sucrose?

Sucrose on hydrolysis gives an equimolar mixture of D-(+)-glucose and D-(-)-fructose. These two monosaccharides are held together by a glycosidic linkage between C−1 of α-glucose and C−2 of β-fructose.

What happens during acid hydrolysis?

In organic chemistry, acid hydrolysis is a hydrolysis process in which a protic acid is used to catalyze the cleavage of a chemical bond via a nucleophilic substitution reaction, with the addition of the elements of water (H2O). For example, in the conversion of cellulose or starch to glucose.

What is the role of acid hydrolysis in feulgen reaction?

Acid hydrolysis removes purin bases from the DNA, thereby unmasking free aldehyde groups. The aldehyde groups then react with Schiff’s reagent, which results in the purple staining. RNA is not hydrolyzed by the HCl treatment and, thus, the reaction is DNA-specific.

Why is RNA readily hydrolyzed by acid solutions?

RNA is susceptible to this base-catalyzed hydrolysis because the ribose sugar in RNA has a hydroxyl group at the 2′ position. This feature makes RNA chemically unstable compared to DNA, which does not have this 2′ -OH group and thus is not susceptible to base-catalyzed hydrolysis.

What is feulgen positive?

Feulgen stain is a staining technique discovered by Robert Feulgen and used in histology to identify chromosomal material or DNA in cell specimens. It is darkly stained. It depends on acid hydrolysis of DNA, therefore fixating agents using strong acids should be avoided.

Why is RNA so fragile?

While DNA contains deoxyribose, RNA contains ribose, characterised by the presence of the 2′-hydroxyl group on the pentose ring (Figure 5). This hydroxyl group make RNA less stable than DNA because it is more susceptible to hydrolysis. Some viruses use RNA instead of DNA as their genetic material.