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Why is PCR so important?

PCR has become an important tool for medical diagnosis. PCR can detect and identify bacteria and viruses that cause infections such as tuberculosis, chlamydia, viral meningitis, viral hepatitis, HIV, cytomegalovirus and many others. PCR is used to amplify the gene, which is then sequenced to look for mutations.

Which of the following is not a function of reverse transcriptase?

Which of the following is not a function of reverse transcriptase? Explanation: Reverse transcription has a high error rate due to no proofreading activity. Thus the reverse transcriptase that facilitates reverse transcription has no exonuclease activity.

What is the normal role of reverse transcriptase?

Reverse transcriptase copies RNA back to DNA. Reverse transcriptase drives the opposite way in molecular processes in cells, converting RNA back to DNA. Although it is very different from the normal process, reverse transcriptase is an important enzyme. It is needed for function in viruses, eukaryotes and prokaryotes.

What is the distinctive feature of reverse transcriptase?

What is the distinctive feature of reverse transcriptase? DNA fragments with single-stranded ends.

What is the function of reverse transcriptase mastering biology?

What is the function of reverse transcriptase? it catalyzes the formation of DNA from an RNA template.

Do eukaryotic cells have reverse transcriptase?

Abstract. Genes encoding reverse transcriptases (RTs) are found in most eukaryotes, often as a component of retrotransposons, as well as in retroviruses and in prokaryotic retroelements.

Do humans encode reverse transcriptase?

The human LINE-1 ORF2, which encodes reverse transcriptase, was inserted into a baculovirus shuttle vector and expressed in Sf 21 cells. An immunoreactive polypeptide (149kDa) synthesized by infected cells had reverse transcriptase activity.

What organisms have reverse transcriptase?

Reverse transcriptase, discovered in 1970 in retroviruses, has until recently been found only in eukaryotic organisms. Recently it was shown to occur in two groups of bacteria: myxobacteria and Escherichia coli.

What proteins come packaged in the virus?

The Pol protein of human immunodeficiency virus type 1 (HIV-1) harbours the viral enzymes critical for viral replication; protease (PR), reverse transcriptase (RT), and integrase (IN).

Why is it more difficult to develop a vaccine for a retrovirus?

“One of the reasons why it has been so difficult to make an AIDS vaccine is that the virus infects the very cells of the immune system that any vaccine is supposed to induce,” senior author Dr. Guido Silvestri, chief of microbiology and immunology at Yerkes National Primate Research Center said in a statement.

Is polio the same as smallpox?

Unfortunately, polio differs from smallpox in another crucial way. Approximately 95% of those infected either don’t display any symptoms or only display generic ones such as fever and headache. This means the type of disease tracking that officials used to detect smallpox epidemics is impossible for polio.

Why is it difficult to develop vaccines for retroviruses quizlet?

why is it more difficult to develop a vaccine for a retrovirus? HIV virus uses the reverse transcriptase and RNA method. because the single stranded genetic material develops mutations more frequently than DNA viruses. what proteins come packaged in the HIV virus?

Which virus has been eradicated?

A child suffering from smallpox. In 1980, the World Health Organization announced the global eradication of smallpox. It is the only human disease to be eradicated worldwide.

What diseases are close to being eradicated?

Diseases we could eradicate

  • Polio.
  • Guinea worm disease (dracunculiasis)
  • Lymphatic filariasis.
  • Measles, mumps and rubella.
  • Cysticercosis.